Establishment and Analysis of Environmental Microbial Library in Microbiology Testing Laboratory
Objective:To establish the environmental microbial library of microbiology testing laboratory and provide guidance for contamination traceability.Method:Sedimentation bacteria,planktonia bacteria and surface microorganisms were collected from different parts and personnel of the microbiology testing laboratory for 12 months.16S rDNA sequence alignment was used to identify contaminated microorganisms.Result:A total of 109 strains of microorganisms were collected.The contaminating microorganisms were mainly distributed in 7 genera,of which the Staphylococcus accounted for 37.61%,the Bacillus accounted for 24.77%,the Micrococcus accounted for 13.76%,the Kocuria accounted for 9.18%,the Moraxella accounted for 3.67%,the Sphingomonas accounted for 3.67%and the fungi accounted for 7.34%.Conclusion:The environmental microbial library of the microbiology testing laboratory has been preliminarily established,and the distribution and composition of the microorganisms in the microbiology testing laboratory have been basically understood,which has guiding significance for the environmental risk monitoring of the laboratory in the later stage.
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