Study on the Comparison and Optimization of DNA Extraction Methods of Snail Meat Soup Packets
In order to effectively extract the DNA of snail meat soup packets from prepackaged Liuzhou river snail rice noodles,the effects of four methods of DNA extraction,namely,SDS method,CTAB method,DNA extraction kit for deep-processed food(precipitation method),and DNA extraction kit for food(over-column method),were compared with each other using DNA concentration,purity,and validity as the indexes,and the method with the better extraction effect was selected to be optimized and improved.The results showed that the extraction effect of CTAB method was better among the four methods,and after the optimization of the sample pretreatment and extraction process of the method,the extracted DNA concentration reached 110.800 ng·μL-1,and the purity indexes were 1.803 for A260/A280 and 1.670 for A260/A230,which were suitable for further molecular biology testing and laid the foundation for further source component.
deoxyribonucleic acid extractionLiuzhou river snail rice noodlesnail meat soup packets
NETL
NSTL
万方数据
