超高效液相色谱-三重四极杆质谱联用仪测定葡萄中的两种甜味剂
Determination of 2 Sweeteners in Grape by UPLC-MS/MS
曹佳 1陈旭晋 1周锋杰 1陆宇阳1
作者信息
- 1. 苏州市农产品质量安全监测中心,江苏 苏州 215000
- 折叠
摘要
建立一种使用超高效液相色谱-三重四极杆质谱联用仪对葡萄样品中的甜蜜素(环己基氨基磺酸钠)和纽甜等甜味剂进行定性分析和定量检测的方法.样品经提取后上机,采用多反应监测模式,负离子扫描.在1～50 ng·mL-1 呈良好的线性关系,加标回收率为 87.6%～96.8%,相对标准偏差≤5.8%.该方法具有前处理方便快捷、检测效率高、抗干扰能力强、准确度高、重复性好等特点,可作为新鲜葡萄样品中部分甜味剂的检测方法,也可为检测其他固体样品提供参考.
Abstract
Establishing a method for the qualitative analysis and quantitative detection of sweeteners such as sodium cyclamate and neotame in grape samples using a ultra high performance liquid chromatography triple quadrupole mass spectrometer system.The samples are prepared and injected into the instrument after extraction,utilizing a multiple reaction monitoring mode and negative ion scanning.There is a good linear relationship in 1～50 ng·mL-1,with a spike recovery rate of 87.6%to 96.8%and the relative standard deviation≤5.8%.This method is characterized by its simplicity in sample preparation,high detection efficiency,accuracy,and good repeatability.It can be used for the detection of certain sweeteners in fresh grape samples.
关键词
甜蜜素/纽甜/葡萄/超高效液相色谱-三重四极杆质谱联用仪Key words
sodium cyclamate/neotame/grape/ultra high performance liquid chromatography triple quadrupole mass spectrometer引用本文复制引用
出版年
2024
NETL
NSTL
万方数据