Rapid Determination of Benzimidazoles Residues in Fruit Juice by High Performance Liquid Chromatography Coupled with On-line Solid Phase Extraction
In this research,a new method for on-line purification of fruit juice samples and determination of carbendazim,thiophanate methyl and thiabendazole residues by high performance liquid chromatography(HPLC)with on-line solid phase extraction(SPE)was developed.The juice samples were centrifuged and fed directly into a HPLC analyzer.After purification and enrichment by extraction column,the target compounds were separated by C18 column and detected by diode array detector and fluorescence detector respectively.The results showed that with the diode array detector,the calibration curves of carbendazim,thiophanate methyl and thiabendazole had a good linear relationship in the range of 0.01～2.00 μg·mL-1,the limit of detection reached 0.01 μg·mL-1.With the fluorescence detector,the calibration curves of carbendazim,thiophanate methyl and thiabendazole had a good linear relationship in the range of 0.005～2.000 μg·mL-1,the limit of detection reached 0.005 μg·mL-1.The recoveries were in the range of 89.4%～106.9%,with the relative standard deviations of 0.2%～5.4%.The method was simple,rapid,accurate and reproducible,and suitable for the rapid determination of carbendazim,thiophanate methyl and thiabendazole residues in fruit juice.
high performance liquid chromatographyon-line solid phase extractionjuicebenzimidazoles
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