Determination a Variety of Organotin Compounds in Foodstuffs of Plant Origin by High Performance Liquid Chromatography-Tandem Mass Spectrometry
The samples were extracted with formic acid acetonitrile(1%formic acid)and formic acid methanol(1%formic acid),respectively.The supernatant extract was dehydrated and layered using a mixed salt precipitation reagent.The supernatant was mixed with water in a ratio of 2∶3(volume ratio),and then subjected to membrane filtration and machine detection.Using 0.2 g·L-1 ammonium acetate aqueous solution(containing 0.1%formic acid)-0.1%formic acid acetonitrile as the mobile phase,gradient elution was performed at a flow rate of 0.3 mL·min-1.A benzene chromatography column was used for liquid phase separation,positive ion mode detection,and external standard quantification.The results showed that among the five substrates of strawberries,apples,citrus,grapes,and cucumbers,seven organic tin compounds had a good linear relationship,with correlation coefficients≥0.995;the detection limit of the method is 0.2～4 μg·kg-1,lower limit of quantification is 0.5～10 μg·kg-1;at three levels of concentration addition,the average recovery rates of seven organotin compounds were 68.1%～103.1%,with a relative standard deviation of 1.00%to 11.36%.This method is accurate,sensitive,and fast,and is suitable for quantitative analysis of various organic tin compounds in plant-based foods.
high performance liquid chromatography-tandem mass spectrometryorganotin compoundsfoodstuffs of plant origin
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