摘要
保健食品中总皂苷的测定方法存在标准曲线线性差、样品检测数据重现性差以及回收率偏低的问题.本文以该方法为基础,将样品提取液净化时的洗脱液由 70%乙醇改为 70%甲醇、洗脱液的浓缩方式由水浴挥干改为氮吹至干.方法改进后,检测耗时、标准曲线线性关系、样品测定结果重现性以及回收率明显改善.
Abstract
There are problems with the determination method of total saponins in health food,such as poor linearity of the standard curve,poor repetition of sample detection data,and low recovery rate.Based on this method,this article changes the elution solution during sample extraction purification from 70%ethanol to 70%methanol,and the concentration method of the elution solution from water bath evaporation to nitrogen blowing to dryness.After the improvement of the method,the detection time,linearity of the standard curve,reproducibility of sample determination results,and recovery rate of the project have significantly improved.
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