Determination of Amantadine and Fipronil in Eggs by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry
Objective:To establish a method for the determination of amantadine and fipronil in eggs by ultra performance liquid chromatography-tandem mass spectrometry.Method:The sample solution was added with internal standard working solution,extracted with acetonitrile and purified by QuEChERS purification tube.Amantadine was quantified by positive ion scanning and internal standard method;fipronil was quantified by negative ion scanning and external standard method.Result:The linear relationship between amantadine and fipronil was good in the concentration range of 0.20～20.00 ng·mL,the recovery rate of the tested substance is 85.0%～103.3%,and the precision is 1.6%～8.9%,the quantitative limit of amantadine and fipronil in this method is 2 μg·kg-1 and 5 μg·kg-1 respectively.Conclusion:This method has high accuracy,high sensitivity,good stability and simple operation,and can quickly and effectively determine the contents of amantadine and fipronil in eggs.
ultra-high performance liquid chromatography tandem mass spectrometryeggsamantadinefipronilcontent determination
NETL
NSTL
万方数据
