Detection of Soybean Isoflavone Content by Ultra-High Performance Liquid Chromatography
Objective:To establish an efficient detection method for determining the content of soy isoflavones in soybeans and study the differences in soy isoflavone components.Method:Ultra high performance liquid chromatography was used to detect the content of soy isoflavones in soybeans.A 70%methanol solution was added to the soy sample,and soy isoflavones were extracted by ultrasonic method.The chromatographic column was BEHC C18 column(150 mm×2.1 mm,1.7 μm),the mobile phase is acetonitrile-0.2%acetic acid,the detection wavelength is 260 nm,the column temperature is 30℃,and the flow rate is 0.15 mL·min-1.Result:The linear relationship,precision,and stability of the four soybean isoflavones were good,with an average recovery rate of 97.6%to 98.6%.The content of daidzin,the isoflavone component of soybeans,is the highest,while the content of daidzein is the lowest.The specific content is daidzin 352.0 μg·g-1,genistein 88.0 μg·g-1,genistein 25.8 μg·g-1,daidzein 19.5 μg·g-1.Conclusion:Ultra high performance liquid chromatography has the advantages of simple operation,fast detection speed,and high sensitivity,and can be used for the detection of soy isoflavone content in soybeans.
ultra high performance liquid chromatographydetectionsoybeanssoy isoflavones
NETL
NSTL
万方数据
