超高效液相色谱法检测大豆异黄酮含量
Detection of Soybean Isoflavone Content by Ultra-High Performance Liquid Chromatography
靳颖1
作者信息
- 1. 黄河交通学院,河南 焦作 454950
- 折叠
摘要
目的:建立一种测定大豆中大豆异黄酮含量的高效检测方法,研究大豆中大豆异黄酮成分的差异.方法:采用超高效液相色谱法检测大豆中大豆异黄酮含量,在大豆样品中加入 70%甲醇溶液,超声波法提取大豆异黄酮,色谱柱为BEHC C18 柱(150 mm×2.1 mm,1.7 μm),流动相为乙腈-0.2%乙酸,检测波长为260 nm,柱温为 30℃,流速为 0.15 mL·min-1.结果:4 种大豆异黄酮成分的线性关系、精密度、稳定性良好,平均回收率为 97.6%～98.6%.大豆中大豆异黄酮成分大豆苷含量最高,大豆苷元含量最低,具体含量为大豆苷352.0 μg·g-1,染料木苷88.0 μg·g-1,染料木素25.8 μg·g-1,大豆苷元19.5 μg·g-1.结论:超高效液相色谱法操作简单、检测速度快、灵敏度高,可用于大豆中大豆异黄酮含量检测.
Abstract
Objective:To establish an efficient detection method for determining the content of soy isoflavones in soybeans and study the differences in soy isoflavone components.Method:Ultra high performance liquid chromatography was used to detect the content of soy isoflavones in soybeans.A 70%methanol solution was added to the soy sample,and soy isoflavones were extracted by ultrasonic method.The chromatographic column was BEHC C18 column(150 mm×2.1 mm,1.7 μm),the mobile phase is acetonitrile-0.2%acetic acid,the detection wavelength is 260 nm,the column temperature is 30℃,and the flow rate is 0.15 mL·min-1.Result:The linear relationship,precision,and stability of the four soybean isoflavones were good,with an average recovery rate of 97.6%to 98.6%.The content of daidzin,the isoflavone component of soybeans,is the highest,while the content of daidzein is the lowest.The specific content is daidzin 352.0 μg·g-1,genistein 88.0 μg·g-1,genistein 25.8 μg·g-1,daidzein 19.5 μg·g-1.Conclusion:Ultra high performance liquid chromatography has the advantages of simple operation,fast detection speed,and high sensitivity,and can be used for the detection of soy isoflavone content in soybeans.
关键词
超高效液相色谱法/检测/大豆/大豆异黄酮Key words
ultra high performance liquid chromatography/detection/soybeans/soy isoflavones引用本文复制引用
出版年
2024
NETL
NSTL
万方数据