QuEChERS-液相色谱串联质谱法检测畜禽肉中263种农药残留
Determination of 263 Pesticide Residues in Livestock and Poultry Meat by QuEChERS-LC-MS/MS
孙银峰 1靳智 1崔芳芳 1姜志虎1
作者信息
- 1. 青岛元信检测技术有限公司,山东 青岛 266000
- 折叠
摘要
建立了一种快速测定畜禽肉中 263 种农药残留的液相色谱-串联质谱检测方法.本实验中,样品加入乙腈和QuEChERS萃取盐包(1.5 g醋酸钠+6 g无水硫酸镁)提取后,通过QuEChERS净化包(900 mg无水硫酸镁、150 mg N-丙基乙二胺、150 mg十八烷基硅烷)净化,超高效液相色谱-串联质谱测定,基质标准曲线定量.结果表明,263 种农药在 5～200 μg·L-1 浓度范围内线性相关系数均大于 0.99,方法的定量下限为0.010 mg·kg-1,样品平均加标回收率为 60.6%～119.1%,相对标准偏差小于 15%(n=6).该方法前处理简单、准确度和灵敏度高,适合动物源性食品中的农药残留的快速筛查和定量检测,适用于日常检测工作.
Abstract
A rapid method for the determination of 263 pesticide residues in livestock and poultry meat by liquid chromatography-tandem mass spectrometry(LC-MS/MS)was developed.In the experiment,the sample was extracted with acetonitrile and QuEChERS extraction salt bag(1.5 g sodium acetate+6 g anhydrous magnesium sulfate),then purified with QuEChERS purification bag(900 mg anhydrous magnesium sulfate,150 mg N-propylethylenediamine,150 mg octadecylsilane),determined by ultra performance liquid chromatography-tandem mass spectrometry,and quantified by matrix standard curve.The results showed that the calibration curves of 263 pesticides were linear in the range of 5～200 μg·L-1,and the correlation coefficients were all above 0.99.The lower limit of quantification for the method is 0.010 mg·kg-1,the average recoveries were in the range of 60.6%～119.1%,and the relative standard deviations were less than 15%(n=6).The method is simple in pretreatment,high in accuracy and sensitivity,suitable for rapid screening and quantitative detection of pesticide residues in animal-derived foods,and suitable for routine detection.
关键词
液相色谱串联质谱法(LC-MS/MS)/QuEChERS/农药残留Key words
liquid chromatography-tandem mass spectrometry(LC-MS/MS)/QuEChERS/pesticide residues引用本文复制引用
出版年
2024
NETL
NSTL
万方数据