液质联用法测定婴幼儿奶粉中左旋肉碱含量不确定度评定
Evaluation of Uncertainty in the Determination of L-carnitine Content in Infant Formula by Liquid Chromatography-mass Spectrometry
张伟 1杨敏 1李永忠 2夏敏 2易佳容3
作者信息
- 1. 湖南口味王集团有限责任公司,湖南 益阳 413000;湖南中益食品化工检测院,湖南 益阳 413000
- 2. 湖南中益食品化工检测院,湖南 益阳 413000
- 3. 湖南口味王集团有限责任公司,湖南 益阳 413000
- 折叠
摘要
本文介绍了采用液质联用同位素内标法测定婴幼儿奶粉中左旋肉碱含量的不确定度评价方法,通过对样品称量、稀释体积和浓度带入的不确定度分别进行评定,最终得到样品中左旋肉碱含量的相对标准不确定度和扩展不确定度.结果表明,婴幼儿奶粉中,左旋肉碱浓度的不确定度主要来源于标准曲线的测定.因此,在实验过程中,相关人员应严格控制实验条件,保证标准曲线的准确度.
Abstract
This article introduces an uncertainty evaluation method for the determination of L-carnitine content in infant formula using liquid chromatography-mass spectrometry(LC-MS)isotope internal standard method.By evaluating the uncertainty brought in by sample weighing,dilution volume,and concentration,the relative standard uncertainty and extended uncertainty of L-carnitine content in the sample are finally obtained.The results indicate that the uncertainty of L-carnitine concentration in infant formula mainly comes from the determination of the standard curve.Therefore,during the experiment,relevant personnel should strictly control the experimental conditions to ensure the accuracy of the standard curve.
关键词
不确定度/左旋肉碱/婴幼儿奶粉/液质联用Key words
uncertainty/L-carnitine/infant formula/liquid chromatography-mass spectrometry引用本文复制引用
出版年
2024
NETL
NSTL
万方数据