Validation of Qualitative Methods for Real-time Fluorescence PCR Detection of Mouse Derived Components
This article explores the feasibility and applicability of GB/T 38164-2019"Real time Fluorescence PCR Method for Detection of Common Livestock and Poultry Animal Derived Ingredients"in the detection of mouse derived ingredients in meat food,aiming to provide reference for the method validation of animal derived ingredients.The results indicate that the experimental method of GB/T 38164-2019 has good specificity for rats and can accurately detect rat derived components in meat products.However,this standard method is not applicable for detecting mouse derived components in meat products and requires further optimization.