In order to improve the nutritional value of biscuits,a biscuit was developed from mushroom,and the formula of mushroom biscuit was optimized by single factor test and orthogonal test.The results showed that the best formula of mushroom biscuits was 10 g of mushroom powder,36 g of butter,18 g of granulated sugar,0.4 g of baking soda and 5 g of milk.The mushroom cookies made according to this formula are crispy and overflowing in aroma,which is highly loved by people and enrich the variety of mushroom products.