固相萃取-液相色谱法同时测定复杂食品中的5种添加剂
Determination of 5 Kinds Additives in Complex Food by Solid Phase Extraction Combined with Liquid Chromatography
杨鲁琼 1孙志伟 2汪腊云 1朱晓薇1
作者信息
- 1. 绿城农科检测技术有限公司,浙江 杭州 310052
- 2. 四维生态科技(杭州)有限公司,浙江 杭州 310052
- 折叠
摘要
本文采用ProElut FA固相萃取的前处理方式,建立了同时测定复杂食品基质中安赛蜜、糖精钠、苯甲酸、山梨酸和脱氢乙酸的液相色谱检测方法.样品采用水和磷酸盐缓冲液提取,ProElut FA固相萃取柱净化,紫外检测器测定.结果表明,5 种添加剂在 0.25～50.00 mg·L-1 线性良好(相关系数≥0.999 5),方法定量限为0.401～0.481 mg·kg-1,加标回收率为 72.6%～109.0%.本方法能有效减少杂质干扰,提高定量检测的准确度,减少色谱柱损耗.
Abstract
This article adopts the pre-treatment method of ProElut FA solid-phase extraction to establish a liquid chromatography detection method for simultaneous determination of acesulfame,saccharin sodium,benzoic acid,sorbic acid,and dehydroacetic acid in complex food matrices.The samples were extracted using water and phosphate buffer,purified using a ProElut FA solid-phase extraction column,and determined using a UV detector.The results showed that the five additives had good linearity in the range of 0.25～50.00 mg·L-1(correlation coefficient≥0.999 5).The limits of quantitation were 0.401～0.481 mg·kg-1,and the recoveries were 72.6%～109.0%.This method can effectively reduce the interference of impurities,improve the accuracy of quantitative detection,and reduce the loss of chromatographic column.
关键词
防腐剂/甜味剂/液相色谱/固相萃取/复杂食品Key words
preservatives/sweeteners/liquid chromatography/solid phase extraction/complex food引用本文复制引用
出版年
2024
NETL
NSTL
万方数据