荧光光度法测定婴幼儿米粉中的亚硝酸盐
Determination of Nitrite in Infant Rice Flour by Fluorescence Spectrophotometry
范超 1赵涛 1贺思璐 1陈建花 1邓卫芳 1肖盼1
作者信息
- 1. 湖南英氏营养食品有限公司,湖南 长沙 410000
- 折叠
摘要
目的:建立一种荧光光度法测定婴幼儿米粉中的亚硝酸盐.方法:米粉样品经 60℃超声萃取,同时酶解米粉样品中的淀粉,用饱和氯化钠溶液去除样品中的脂肪和沉淀蛋白质,加入活性炭吸附消除样品浊度或色度干扰,过滤后取上清液加入2,3-二氨基萘生成2,3-萘三唑.采用荧光光度计进行检测,激发波长365 nm,发射波长 450 nm.结果:亚硝酸盐在 0～0.10 mg·L-1 具有良好线性,相关系数为 0.999 6,检出限和定量限分别为 0.028 mg·kg-1 和 0.085 mg·kg-1,回收率在 94.0%～97.7%,相对标准偏差在 0.17%～0.84%.结论:本研究建立了一种简单、灵敏、准确的荧光光度法,可用于测定婴幼儿米粉中的亚硝酸盐含量.
Abstract
Objective:A fluorescence spectrophotometric method was established for the determination of nitrite in infant rice flour.Method:The rice flour sample was ultrasonically extracted at 60℃,and the starch in the rice flour sample was hydrolyzed at the same time.The fat and precipitated protein in the sample were removed with saturated sodium chloride solution.The activated carbon adsorption was added to eliminate the turbidity or chromaticity interference of the sample.After filtration,the supernatant was added with 2,3-diaminonaphthalene to form 2,3-naphthalene triazole.The excitation wavelength was 365 nm and the emission wavelength was 450 nm.Result:Nitrite had a good linearity in the range of 0～0.10 mg·L-1,the correlation coefficient was 0.999 6,the limit of detection and limit of quantitation were 0.028 mg·kg-1 and 0.085 mg·kg-1,respectively.The recovery rate was 94.0%～97.7%,and the relative standard deviation was 0.17%～0.84%.Conclusion:In this study,a simple,sensitive and accurate fluorescence spectrophotometry was established for the determination of nitrite content in infant rice flour.
关键词
亚硝酸盐/婴幼儿米粉/荧光光度法/谷物辅助食品Key words
nitrite/infant rice flour/fluorescence spectrophotometry/cereal auxiliary food引用本文复制引用
基金项目
湖南省科技创新项目(2023ZJ1070)
出版年
2024
NETL
NSTL
万方数据