Lineweaver-Burk双倒数法研究姜酚对α-淀粉酶和α-葡萄糖苷酶的动力学抑制活性
Lineweaver-Burk Double Reciprocal Method to Study the Kinetic Inhibitory Activity of Gingerol on α-Amylase and α-Glucosidase
徐菁 1孙浩强 2张校志3
作者信息
- 1. 贵阳信息科技学院,贵州 贵阳 550025
- 2. 贵阳学院体育学院,贵州 贵阳 550005
- 3. 贵州商学院,贵州 贵阳 550014
- 折叠
摘要
用Lineweaver-Burk双倒数法研究姜酚对α-淀粉酶和α-葡萄糖苷酶的动力学抑制类型.结果表明,姜酚对α-淀粉酶和α-葡萄糖苷酶均有明显的抑制效果,抑制类型分别为竞争型抑制和混合型抑制,其抑制常数Kic1、Kic2 分别为 3.88 和 2.67.与α-淀粉酶相比,姜酚与α-葡萄糖苷酶结合的亲和力更好.
Abstract
The Lineweaver-Burk double reciprocal method was used to study the kinetic inhibition type of gingerol on α-amylase and α-glucosidase,and its interaction force was analyzed through thermodynamic parameters.The results showed that gingerol had obvious inhibitory effects on α-amylase and α-glucosidase.The inhibition types were competitive inhibition and mixed inhibition,respectively.The inhibition constants Kic1 and Kic2 were 3.88 and 2.67,respectively.Compared with α-amylase,the binding affinity of gingerol to α-glucosidase was better.
关键词
姜酚/α-淀粉酶/α-葡萄糖苷酶Key words
gingerol/α-amylase/α-glucosidase引用本文复制引用
出版年
2024
NETL
NSTL
万方数据