Lineweaver-Burk Double Reciprocal Method to Study the Kinetic Inhibitory Activity of Gingerol on α-Amylase and α-Glucosidase
The Lineweaver-Burk double reciprocal method was used to study the kinetic inhibition type of gingerol on α-amylase and α-glucosidase,and its interaction force was analyzed through thermodynamic parameters.The results showed that gingerol had obvious inhibitory effects on α-amylase and α-glucosidase.The inhibition types were competitive inhibition and mixed inhibition,respectively.The inhibition constants Kic1 and Kic2 were 3.88 and 2.67,respectively.Compared with α-amylase,the binding affinity of gingerol to α-glucosidase was better.
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