响应面法优化河蚬多肽的制备工艺
Response Surface Methodology to Optimization of Prearation Process of Polypeptide from Corbicula fluminea
刘依云 1李思默 1刘思宇 1岳梅 1毛璇 1陈洪彬1
作者信息
- 1. 泉州师范学院海洋与食品学院,福建 泉州 362000
- 折叠
摘要
为实现河蚬的高值化利用,本研究采用酶法制备河蚬多肽.以乙醇脱氢酶(Alcohol Dehydrogenase,ADH)激活率为指标,采用单因素和响应面法优化酶解工艺参数.结果表明,在料液比 1∶130(g∶mL)、酶解时间 140 min、酶解温度 45℃的条件下,河蚬多肽对ADH的激活率为 77.21%±0.82%.该研究为河蚬的精深加工及高值化开发利用提供了理论依据.
Abstract
In order to realize the high-value utilization of Corbicula fluminea,this study utilized an enzymatic method to prepare polypeptide from Corbicula fluminea.The alcohol dehydrogenase(ADH)activation rate were used as an indicators,and the enzymatic process parameters were optimized using one-factor and response surface.The results showed that under the conditions of solid to liquid ratio 1∶130(g∶mL),enzymolysis time 140 min and enzymolysis temperature 45℃,the activation rate of polypeptide on ADH was 77.21%±0.82%,the ADH activation rate of polypeptide from Corbicula fluminea was 77.21%±0.82%.This study provides a theoretical basis for the deep processing and high value utilization of Corbicula fluminea.
关键词
河蚬/酶解/工艺优化/ADH激活率/多肽Key words
Corbicula fluminea/enzymolysis/optimization of process/ADH activation rate/polypeptide引用本文复制引用
出版年
2024
NETL
NSTL
万方数据