Response Surface Methodology to Optimization of Prearation Process of Polypeptide from Corbicula fluminea
In order to realize the high-value utilization of Corbicula fluminea,this study utilized an enzymatic method to prepare polypeptide from Corbicula fluminea.The alcohol dehydrogenase(ADH)activation rate were used as an indicators,and the enzymatic process parameters were optimized using one-factor and response surface.The results showed that under the conditions of solid to liquid ratio 1∶130(g∶mL),enzymolysis time 140 min and enzymolysis temperature 45℃,the activation rate of polypeptide on ADH was 77.21%±0.82%,the ADH activation rate of polypeptide from Corbicula fluminea was 77.21%±0.82%.This study provides a theoretical basis for the deep processing and high value utilization of Corbicula fluminea.
Corbicula flumineaenzymolysisoptimization of processADH activation ratepolypeptide
NETL
NSTL
万方数据
