动物源性食品中硝基呋喃类代谢物残留量检测方法的验证
Validation of Methods for Determination of Nitrofuran Metabolites Residues in Food of Animal Origin
吕婷 1邬杰 1刘鑫 1郭兰 1王建军 1李丹阳1
作者信息
- 1. 包头市农畜产品质量安全中心,内蒙古 包头 014000
- 折叠
摘要
本文对《动物源性食品中硝基呋喃类药物代谢物残留量检测方法 高效液相色谱/串联质谱法》(GB/T 21311-2007)进行方法验证.结果表明,1-氨基-乙内酰脲、5-吗啉甲基-3-氨基-2-噁唑烷基酮、3-氨基-2-噁唑酮、氨基脲这 4 种硝基呋喃类代谢物在 0.50～20.00 ng·mL-1 浓度范围内(内标浓度 10 ng·mL-1)有良好的线性关系,相关系数＞0.99,平均回收率在 98.6%～102.0%,相对标准偏差在 0%～1.140%.该方法的灵敏度均满足标准方法中规定的要求.
Abstract
This paper verifies the method of GB/T 21311-2007.The results showed that the 4 nitrofuran metabolites,including 1-amino-hydantoin,5-morpholinomethyl-3-amino-2-oxalidinone,3-amino-2-oxalidinone and semicarbazide,had a good linear relationship in the range of 0.50～20.00 ng·mL-1(internal standard concentration 10 ng·mL-1),the correlation coefficient was＞0.99,and the average recovery rate was 98.6%～102.0%.The relative standard deviation is 0%～1.140%.The sensitivity of the method meets the requirements specified in the standard method.
关键词
硝基呋喃类代谢物/高效液相色谱-串联质谱法/方法验证Key words
nitrofuran metabolite/high performance liquid chromatography-tandem mass spectrometry/method validation引用本文复制引用
出版年
2024
NETL
NSTL
万方数据