UPLC-MS/MS法测定黄瓜中肟菌酯及其代谢物肟菌酸残留量
Determination of Residual Levels of Trifloxystrobin and Its Metabolite Trifloxystrobin Acid in Cucumis sativus by UPLC-MS/MS Method
袁园 1黄思聪 1韩飞2
作者信息
- 1. 肇庆市食品检验所,广东 肇庆 526060
- 2. 汕头职业技术学院,广东 汕头 515078
- 折叠
摘要
采用QuEChERS技术对黄瓜样品进行萃取,经C18 净化和BEH C18 色谱柱分离,采用超高效液相色谱-串联质谱法(Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry,UPLC-MS/MS),测定黄瓜中肟菌酯及其代谢物肟菌酸残留量.结果表明,在 1.0～50.0 μg·L-1,肟菌酯及其代谢物肟菌酸的质量浓度与信号响应的峰面积具有良好的线性关系,相关系数R2≥0.999 7;肟菌酯及其代谢物肟菌酸的检出限为0.05～0.08 μg·kg-1,定量限为0.2～0.3 μg·kg-1;加标回收率为87.3%～101.7%,相对标准偏差为1.1%～3.2%.
Abstract
QuEChERS technology is utilized to extract the Cucumis sativus samples are extracted by QuEChERS technique,and purified by C18 purification and separated by BEH C18 chromatography column,ultra-high performance liquid chromatography-tandem mass spectrometry is used to determine the residual levels of trifloxystrobin and its metabolite trifloxystrobin acid in Cucumis sativus.The results show that in the range of 1.0～50.0 μg·L-1,the mass concentration of trifloxystrobin and its metabolite trifloxystrobin acid had a good linear relationship with the peak area of the signal response,and the correlation coefficient(R2)is 0.999 7 or higher.The limit of detection of trifloxystrobin and its metabolite trifloxystrobin acid is 0.05～0.08 μg·kg-1,and the limit of quantitation is 0.2～0.3 μg·kg-1.The standard recovery is 87.3%～101.7%,the relative standard deviation is 1.1%～3.2%.
关键词
超高效液相色谱-串联质谱/肟菌酯/肟菌酸/黄瓜Key words
ultra-high performance liquid chromatography-tandem mass spectrometry/trifloxystrobin/trifloxystrobin acid/Cucumis sativus引用本文复制引用
基金项目
广东省市场监督管理局科技项目(2023CS13)
出版年
2024
NETL
NSTL
万方数据