Determination of Residual Levels of Trifloxystrobin and Its Metabolite Trifloxystrobin Acid in Cucumis sativus by UPLC-MS/MS Method
QuEChERS technology is utilized to extract the Cucumis sativus samples are extracted by QuEChERS technique,and purified by C18 purification and separated by BEH C18 chromatography column,ultra-high performance liquid chromatography-tandem mass spectrometry is used to determine the residual levels of trifloxystrobin and its metabolite trifloxystrobin acid in Cucumis sativus.The results show that in the range of 1.0～50.0 μg·L-1,the mass concentration of trifloxystrobin and its metabolite trifloxystrobin acid had a good linear relationship with the peak area of the signal response,and the correlation coefficient(R2)is 0.999 7 or higher.The limit of detection of trifloxystrobin and its metabolite trifloxystrobin acid is 0.05～0.08 μg·kg-1,and the limit of quantitation is 0.2～0.3 μg·kg-1.The standard recovery is 87.3%～101.7%,the relative standard deviation is 1.1%～3.2%.
ultra-high performance liquid chromatography-tandem mass spectrometrytrifloxystrobintrifloxystrobin acidCucumis sativus
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