UPLC-MS/MS法测定动源性食品中氯霉素残留量的方法优化研究
Optimization of UPLC-MS/MS Method for Determination of Chlorotoxin Residues in Animal Food
刘娜1
作者信息
- 1. 常德市食品检验所,湖南 常德 415000
- 折叠
摘要
建立一种改良的超高效液质联用法测定动源性食品中氯霉素残留量的检测方法.样品经乙腈溶液提取,利用优化后的前处理法进行净化,经电喷雾离子源,内标法定量分析,并与国标GB 31658.2-2021 进行对比分析.氯霉素在 0.5～20.0 µg·L-1 线性关系良好,相关系数R2＞0.999,方法的检出限为 0.1 µg·kg-1.在3个不同加标浓度水平0.2 µg·kg-1、0.4 µg·kg-1、2.0 µg·kg-1 的样品中回收率均在90%～110%,相对标准偏差＜3%,且阳性样品 7 次重复性测量的相对标准偏差＜3%.该方法前处理简单、结果准确,各项参数都符合相关方法验证的要求,适用于日常对动源性食品中氯霉素残留量的检测.
Abstract
An improved ultra-high performance liquid chromatography-tandem mass spectrometry method was developed for the determination of chloromyces in dynamic foods.The samples were extracted from acetonitrile solution,purified by the optimized pretreatment method,quantitatively analyzed by internal standard method,and compared with the national standard GB 31658.2-2021.Chloromyces in the concentration range of 0.5～20.0 µg·L-1 has a good linear relationship,and the correlation coefficient R2＞0.999,the detection limit of the method was 0.1 µg·kg-1.The recoveries were in the range of 90%～110%.In three different concentrations of 0.2 µg·kg-1,0.4 µg·kg-1 and 2.0 µg·kg-1,the relative standard deviation＜3%,and the relative standard deviation of seven repeated measurements of positive samples＜3%.The pre-processing of this method is simple,the results are accurate and all paramenters meet the requirements of the relevant method verification.It is suitable for the detection of chloramphenicol residues in animal-derived foods.
关键词
超高效液质联用法/动源性食品/氯霉素Key words
ultra-high performance liquid chromatography-tandem mass spectrometry/animal-derived foods/chlorotoxin引用本文复制引用
出版年
2024
NETL
NSTL
万方数据