Exploring the Determination of Acid Orange Ⅱ in Meat Products by High Performance Liquid Chromatography
The content of Acid Orange Ⅱ in meat products was measured by high-performance liquid chromatography,with the mobile phase set as methanol ammonium acetate solution and the UV wavelength adjusted to 484 nm.The test results showed that when the concentration range was between 0.5～10 ug·ml-1,the linear relationship was good(r=0.999 7),and the detection limit and quantification limit were 0.01 mg·kg-1 and 0.05 mg·kg-1,respectively.The recovery rate of the added standard exceeded 95%,and the standard deviation did not exceed 5%.It can quickly detect the content of Acid Orange Ⅱ in meat products with high detection accuracy and high application value.
high performance liquid chromatographymeat productsacid Orange Ⅱextraction
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