QuEChERS-质谱联用仪法测定发酵茶中T-2毒素含量
Determination of T-2 Toxin in Fermented Tea by Quechers-mass Spectrometry
莫海兰 1许秀华1
作者信息
- 1. 梧州市食品药品检验所,广西 梧州 543000
- 折叠
摘要
本文建立QuEChERS-液相质谱联用法测定发酵茶中T-2 毒素的含量.茶叶样品粉碎后,样品经乙腈超声均质提取,HC-C18 去除大部分杂质,采用增强型碳氢键的非极性化合物PSA净化后,使用高性能液相色谱-串联质谱法多反应监测扫描模式进行定性定量分析.T-2 毒素在 5～100 ng·mL-1 浓度范围内呈现良好的线性关系,线性相关系数为 0.999,检出限为 5 μg·kg-1,在 5、20、80 μL三个不同加标水平下,T-2 毒素回收率在 85.01%～87.80%内,相对标准偏差(RSD)为 1.05%.该方法灵敏度高、回收率稳定、检测快速、定量准确.适用于检测发酵茶叶中的T-2 毒素.
Abstract
This article establishes the QuEChERS liquid chromatography-mass spectrometry method for determining the content of T-2 toxin in fermented tea.After crushing the tea sample,the sample was homogenized and extracted by acetonitrile ultrasound,and most impurities were removed by HC-C18.After purification with enhanced non-polar compound PSA with carbon hydrogen bonds,qualitative and quantitative analysis was carried out using high-performance liquid chromatography tandem mass spectrometry multi reaction monitoring scanning mode.The T-2 toxin exhibits a good linear relationship within the concentration range of 5～100 ng·mL-1,with a linear correlation coefficient of 0.999 and a detection limit of 5 μg·kg-1 at 5,20,and 80 μL at three different levels of spiking,the recovery rate of T-2 toxin is within 85.01%～87.80%,with a relative standard deviation(RSD)of 1.05%.This method has high sensitivity,stable recovery rate,fast detection,and accurate quantification.suitable for detecting T-2 toxin in fermented tea.
关键词
T-2毒素/发酵茶/QuEChERS/液相质谱联用法Key words
T-2 toxin/fermented tea/QuEChERS/liquid phase mass spectrometry引用本文复制引用
出版年
2024
NETL
NSTL
万方数据