Determination of T-2 Toxin in Fermented Tea by Quechers-mass Spectrometry
This article establishes the QuEChERS liquid chromatography-mass spectrometry method for determining the content of T-2 toxin in fermented tea.After crushing the tea sample,the sample was homogenized and extracted by acetonitrile ultrasound,and most impurities were removed by HC-C18.After purification with enhanced non-polar compound PSA with carbon hydrogen bonds,qualitative and quantitative analysis was carried out using high-performance liquid chromatography tandem mass spectrometry multi reaction monitoring scanning mode.The T-2 toxin exhibits a good linear relationship within the concentration range of 5～100 ng·mL-1,with a linear correlation coefficient of 0.999 and a detection limit of 5 μg·kg-1 at 5,20,and 80 μL at three different levels of spiking,the recovery rate of T-2 toxin is within 85.01%～87.80%,with a relative standard deviation(RSD)of 1.05%.This method has high sensitivity,stable recovery rate,fast detection,and accurate quantification.suitable for detecting T-2 toxin in fermented tea.
T-2 toxinfermented teaQuEChERSliquid phase mass spectrometry
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