高效液相色谱法测定食品中溴硝醇残留
Determination of Bronopol Residue in Foods by High Performance Liquid Chromatography
彭晓俊 1梁伟华 1秦汉1
作者信息
- 1. 江门海关技术中心,广东 江门 529100
- 折叠
摘要
建立高效液相色谱法测定食品中溴硝醇残留的分析方法.样品经乙醇提取,0.22 μm有机滤膜过滤,Agilent ZORBAX Eclipse XDB-C18 色谱柱分离,高效液相色谱法检测.结果表明,在优化实验条件下,溴硝醇在0.02～10.00 μg·mL-1 具有良好的线性关系,相关系数大于0.999 9.在0.050 μg·mL-1、0.200 μg·mL-1、2.500 μg·mL-1共3个添加水平下,回收率为96.3%～106.0%,相对标准偏差为3.2%～5.8%.该法简单、快速、准确、灵敏度高,可应用于粮食、水果和蔬菜中溴硝醇残留的快速检测.
Abstract
A method for determination of bronopol residue in foods by high performance liquid chromatography was established.The samples were extracted by ethanol,filtered by 0.22 μm organic filter membrane,separated by Agilent ZORBAX Eclipse XDB-C18 column,and detected by high-performance liquid chromatography.Under the optimized experimental conditions,there was a good linear relationship in the range of 0.02～10.00 μg·mL-1 with correlation coefficient greater than 0.999 9.The recoveries on the sample at 3 levels of addition were 96.3%～106.0%,with the relative standard deviations of 3.2%～5.8%.With the advantages of easy and rapid operation,accuracy and high sensitivity,the method was be applied to the determination of bronopol residues in grains,fruits and vegetables.
关键词
高效液相色谱/食品/溴硝醇/甲醇提取Key words
high performance liquid chromatography/food/bronopol/methanol extraction引用本文复制引用
出版年
2024
NETL
NSTL
万方数据