高效液相色谱法测定年糕中二氧化硫脲
Determination of Thiourea Dioxide in Rice Cake by High Performance Liquid Chromatograph
周宁菱 1徐美丽 1孙盼 1吴一明 1吕舟博 1程晓芳 1刘思萌 1黄永芳1
作者信息
- 1. 景德镇市市场监督管理综合检验检测中心,江西 景德镇 333000
- 折叠
摘要
以Amide柱(250 mm×4.6 mm,3.5 μm)为分离柱,以80%乙腈-水溶液为流动相,进行梯度洗脱,在波长 278 nm处检测,建立高效液相色谱法测定年糕中二氧化硫脲含量的分析方法.结果表明,二氧化硫脲在0.2～50.0 mg·L-1 呈良好的线性关系,检出限为 4.00 mg·kg-1,加标回收率为 90.08%～96.73%,相对标准偏差不大于 8.61%.该方法简便快速,实用性强,可用于年糕食品中二氧化硫脲的测定.
Abstract
The Amide column(250 mm×4.6 mm,3.5 μm)was used as stationary phase,gradient elution was performed using 80%acetonitrile aqueous solution as the mobile phase,and detection was performed at a wavelength of 278 nm,to establish an analytical method for determining the content of thiourea dioxide in rice cakes using high-performance liquid chromatography.The results indicated that,the thiourea dioxide exhibited excellent linearity in the range of 0.2～50.0 mg·L-1,the detection limit was 4.00 mg·kg-1.The recovery rates were over the range of 90.08%～96.73%,the relative standard deviation was less than 8.61%.This method is simple,fast,and highly practical,and can be used for the determination of thiourea dioxide in rice cake food.
关键词
年糕/高效液相色谱/二氧化硫脲/分析Key words
rice cake/high performance liquid chromatograph/thiourea dioxide/analysis引用本文复制引用
出版年
2024
NETL
NSTL
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