This paper summarized the establishment background,principles,advantages and disadvantages of the E.coli plate counting method using violet red bile-4-methylumbelliferyl-β-D-glucuronide agar,in order to have a more comprehensive and in-depth understanding of the method,on the basis of which the technical problems existing in the method are discussed,and improvement strategies are proposed,with a view to providing a reference for the revision of the method,so as to carry out the counting of E.coli in food more accurately.
E.coliviolet red bile-4-methylumbelliferyl-β-D-glucuronide agarplate counting methodtechnical problems