Inhibitory Effect of Fermented Soy Protein Probiotic Compound Powder on Helicobacter pylori Infection in Zebrafish
Objective:To study the inhibitory effect of fermented soy protein probiotic compound powder composed of Gastro-AD soy protein powder and Lactobacillus johnsonii LJ88 on Helicobacter pylori(Hp)using zebrafish model.Method:The maximum detection concentration for evaluating the inhibition effect of fermented soy protein probiotic compound powder on zebrafish infection with Hp was determined by administering different concentrations of the compound powder to 4 dpf zebrafish.Then,four concentration groups were set according to 1/8,1/4,1/2 and 1 of the maximum detection concentration to evaluate the effect of fermented soy protein probiotic compound powder on Hp inhibition and intestinal tissue structure.Result:The maximum concentration of fermented soy protein probiotic compound powder for evaluating the inhibitory effect on zebrafish infection with Hp was determined to be 1 000 μg·mL-1.The inhibition rates of Hp in the four concentration groups were 45.38%(p＜0.01),50.37%(p＜0.001),64.35%(p＜0.001)and 64.92%(p＜0.001),respectively,which were better than the positive control group.The intestinal cavity shrinkage of zebrafish in the four concentration groups of fermented soy protein probiotic compound powder was significantly restored;the number of intestinal folds and the morphology of intestinal tissue cells in the four concentration groups were similar to those in the normal control group and better than that in the positive control group,with the efficacy enhancing as the concentration increases.Conclusion:The compound powder composed of Gastro-AD soy protein powder and Lactobacillus johnsonii LJ88 had significant inhibitory effects on Helicobacter pylori.
fermented soy protein probiotic compound powderHelicobacter pylorizebrafishinhibitory effect
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