Determination of Total Sterols in Peony Seed Oil by Gas Chromatography-Mass Spectrometry
The contents of β-sitosterol,ergosterol,campesterol and brassicasterol in peony seed oil were determined by gas chromatography-mass spectrometry.The results showed that ergosterol,brassicasterol,campesterol and β-sitosterol had good linearity in the concentration range of 1～50 μg·mL-1,with correlation coefficients≥0.997 44,recoveries of 75.6%～96.1%and RSD of 0.39%～3.87%.The total sterol contents in peony seed oil were β-sitosterol,ergosterol and campesterol from high to low,and the average content of β-sitosterol was 27.44 g·kg-1.The determination and analysis of the sterol content in peony seed oil can provide data support for relevant enterprises to explore the characteristic functions of peony seed oil and discover characteristic indicators.
gas chromatography-mass spectrometrypeony seed oiltotal sterols
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