Preparation of Allulose Immobilized Enzyme and Optimization of Chromatographic Separation System
D-Allulose is an ideal low-calorie sweetener,mainly prepared by using D-allulose 3-epimerase(DAEase).In order to achieve large-scale and efficient commercial production,Co2+resin was used as immobilized carrier and chitosan solution was cross-linked to DAEase to obtain immobilized enzyme.The chromatographic separation system of immobilized enzyme and gel-type polystyrene calcium chromatographic resin was used to prepare allulose.The key factors affecting the conversion rate were determined and optimized.The results of this study may contribute to the cost reduction of d-Allulose industrial production and provide a potential immobilized enzyme strategy for future large-scale manufacturing of d-Allulose.
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