Research on Biological Enzymatic Extraction Technology of Polygonatum Polysaccharides
Using Rhizoma polygonate as the raw material and Rhizoma polygonate polysaccharide yield as the indicator,the composite enzyme preparation was selected as the optimal enzyme among four enzymes:cellulase,pectinase,β-glucanase,and composite enzyme preparations(high conversion rate saccharifying enzyme,β-glucanase,and pectinase).The L9(34)orthogonal experimental design was used to optimize the process parameters of Rhizoma polygonate polysaccharide biological enzyme extraction.The results showed that the Rhizoma polygonate polysaccharide yield could reach more than 2.5%,when the composite enzyme preparations addition amount was 1.4%,the solid-liquid ratio was 1:20,and treated at 55℃for 100minutes.
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