Study on the Content of Active Components and Inhibition of Xanthine Oxidase Activity in Different Solvent Parts of Ethanol Part of Sunflower Leaves
Sunflower leaf alcohol extract was extracted with petroleum ether,trichloromethane,ethyl acetate and n-butanol.The contents of total flavonoids,total phenols,total saponins and chlorogenic acid in different extraction parts were determined,and the inhibitory activity of xanthine oxidase(XOD)in different parts of sunflower leaf alcohol extract was investigated.The contents of total saponins and total flavonoids in petroleum ether were the highest.The content of total phenol in ethyl acetate was the highest.Chlorogenic acid content of trichloromethane was the highest and the activity of inhibiting XOD was the best.After the chloroform part was purified by macroporous resin,70%ethanol part had the best inhibitory activity on XOD,and the inhibitory type was reversible.
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