Study on Optimization of Ultrasound-Assisted Extraction of Total Flavonoids from Mulberry Leaf Tea and Its Antioxidant Activity
Ultrasonic-assisted extraction of total flavonoids from mulberry leaf tea was used.The extraction process was optimized by single factor test and orthogonal test,and the scavenging ability of total flavonoids in the extract on hydroxyl radicals(·OH)and 1,1-phenyl-2-picrylhydrazyl radicals(DPPH·)was preliminarily studied.The results showed that the optimal extraction process was extraction temperature 50℃,ethanol concentration 50%,and solid-liquid ratio 1∶50(g∶mL).Under this process condition,the average extraction rate of total flavonoids was 5.52%.The extracted total flavonoids had strong antioxidant properties and had obvious scavenging ability on hydroxyl radicals and 1,1-phenyl-2-picrylhydrazyl radicals.