Submerged Fermentation of Ganoderma lucidum Strain and Optimization of Beverage Formulation
Ganoderma lucidum is a precious fungus with high nutritional and medical value.To improve the economy of submerged fermentation of Ganoderma lucidum and the convenient application of culture broth,the medium was formulated with edible raw materials,and the mycelial biomass was used as the evaluation index.The fermented liquid of Ganoderma lucidum was used for beverage preparation,and the formula of fermented beverage was optimized by fuzzy comprehensive evaluation method.The results showed that the optimum medium was 30 g·L-1 sucrose and 15 g·L-1 corn flour,and the mycelial biomass was 6.86 g·L-1.The concentration of exopolysaccharides,polyphenols and flavonoids in the fermentation broth was 430.21 μg·mL-1,26.07 μg·mL-1 and 8.16 μg·mL-1,respectively.The highest sensory score of beverage was obtained when the volume of fermentation broth was 60%,with mass scores of citric acid 0.5%,xanthan gum 0.30%,sucrose 6%,and tartrazine 0.05%in the beverage.
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