Optimization of Protective Agent Formula for Freeze-drying of Lyophilized Bifidobacterium animalis subsp.lactis by Response Surface Methodology
To optimize the formulation of cryoprotectants for lyophilized Bifidobacterium animalis subsp.lactis,the factors of lyoprotectant that have a greater impact on the survival rate of Bifidobacterium animalis subsp.lactis were obtained through the single factor test combined with the statistical analysis of SPSS software.To optimize the formula of lyoprotectant,three factors that have influence on the survival rate in a single factor experiment were selected for response surface analysis based on the survival rate.The research concluded the optimum ofcryoprotectants as follows:The skimmed milk 11.31 g/100 g,trehalose 13.96 g/100 g,glycerol 1.69 g/100 g.By using this process,the optimal cell survival rate was observed to be 93.31%,it had the features of practicability.
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