Optimization of Bi-enzyme Extraction Process for Soybean Protein
Soybean protein possesses high nutritional value,yet the quality of protein may decline by the traditional method of alkaline extraction and acid precipitation.To obtain high-quality soybean protein and increase protein extraction rate,a bi-enzyme stepwise hydrolysis process was employed to extract crude soybean protein.Single factor experiments and orthogonal experimental designs were employed to explore the influence of various factors on protein extraction rate.The results showed that the optimum cellulase enzymatic hydrolysis process was enzymatic pH 4.8,enzymatic temperature 50℃,enzymatic time 2.0 h,and enzymatic addition 0.4%;the optimal alkaline protease enzymatic hydrolysis process was enzymatic pH 9.0,enzymatic temperature 60℃,enzymatic time 5.0 h,and enzymatic addition 0.8%.Under the optimal conditions of bi-enzyme extraction,the extraction rate of crude soybean protein was up to 93.10%.
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