双酶法提取大豆蛋白工艺优化
Optimization of Bi-enzyme Extraction Process for Soybean Protein
李超 1吴冉 1马航 1唐春兰 1白宇涵1
作者信息
- 1. 云南云天化股份有限公司研发中心,云南 昆明 650228
- 折叠
摘要
大豆蛋白营养价值较高,但传统的碱溶酸沉提取工艺会降低蛋白品质.为获得高品质大豆蛋白并提高蛋白提取率,采用双酶法分步水解工艺对大豆粗蛋白进行提取.通过构建单因素实验与正交实验,系统地研究了不同因素对蛋白提取率的影响.研究结果显示,最优的纤维素酶酶解工艺为酶解pH值4.8、酶解温度50℃、酶解时间 2.0 h以及酶添加量 0.4%;最优的碱性蛋白酶酶解工艺为酶解pH值 9.0、酶解温度 60℃、酶解时间5.0 h以及酶添加量 0.8%.在双酶最佳提取条件下,大豆粗蛋白提取率可达 93.10%.
Abstract
Soybean protein possesses high nutritional value,yet the quality of protein may decline by the traditional method of alkaline extraction and acid precipitation.To obtain high-quality soybean protein and increase protein extraction rate,a bi-enzyme stepwise hydrolysis process was employed to extract crude soybean protein.Single factor experiments and orthogonal experimental designs were employed to explore the influence of various factors on protein extraction rate.The results showed that the optimum cellulase enzymatic hydrolysis process was enzymatic pH 4.8,enzymatic temperature 50℃,enzymatic time 2.0 h,and enzymatic addition 0.4%;the optimal alkaline protease enzymatic hydrolysis process was enzymatic pH 9.0,enzymatic temperature 60℃,enzymatic time 5.0 h,and enzymatic addition 0.8%.Under the optimal conditions of bi-enzyme extraction,the extraction rate of crude soybean protein was up to 93.10%.
关键词
大豆蛋白/酶解/提取/纤维素酶/碱性蛋白酶Key words
soybean protein/enzymatic hydrolysis/extraction/cellulase/alkaline protease引用本文复制引用
出版年
2024
NETL
NSTL
万方数据