Determination of Residues of Triclabendazole and Its Metabolite in Cattle and Sheep Fat by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
A method for the determination of residues of triclabendazole and its metabolite ketotriclabendazole in cattle and sheep fat was established using ultra performance liquid chromatography-tandem mass spectrometry.The sample was extracted with acetonitrile,purified with hexane,separated by BEH C18 chromatographic column,gradient elution with acetonitrile and 0.1%formic acid solution,and determined by electrospray negative ion mode.The results showed that there was a good linear relationship(R2＞0.99)between triclabendazole and ketotriclabendazole in the range of 5～500 ng·mL-1;the detection limit and quantification limit of the method were both 0.5 μg·kg-1 and 1 μ g·kg-1;the average recoveries were between 81.6%and 102.3%at the addition levels of 1 μg·kg-1,10 μg·kg-1,and 50 μg·kg-1,and the relative standard deviations within the batch(n=6)and between batches(n=3)were≤14.0%.This method has high accuracy and good precision,which is suitable for determination of residues of triclabendazole and its metabolite in cattle and sheep fat.
cattle and sheep fattriclabendazoleketotriclabendazoleultra performance liquid chromatography-tandem mass spectrometry
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