解淀粉芽孢杆菌产核苷特性初步研究
Preliminary Study on the Characteristics of Nucleoside Production by Bacillus amyloliquefaciens
利佳炜 1徐滕宇 1李沛 2李欣1
作者信息
- 1. 工业发酵省部共建协同创新中心,发酵工程教育部重点实验室,湖北工业大学生命科学与健康工程学院,湖北 武汉 430068
- 2. 安琪酵母股份有限公司酵母功能湖北省重点实验室,湖北 宜昌 443000
- 折叠
摘要
为了探索解淀粉芽孢杆菌在植物基原料中的自然发酵特性,围绕细菌生长、肌苷与鸟苷产量,优化 3 种植物基原料的添加浓度,并确定复合植物基原料添加比例,同时考察最优单一和复合植物基原料发酵过程糖耗、有机酸代谢、酶活力变化和相关基因表达差异.实验结果表明,添加 50 g·L-1 大豆分离蛋白发酵后核苷产量最高,且该组蛋白酶活力最高(252.52 U·mL-1);谷朊粉有利于解淀粉芽孢杆菌具备更强的α-淀粉酶活力,但会产生高质量浓度的乙酸,进而抑制菌体生长;添加 50 g·L-1 植物基原料且谷朊粉和大豆分离蛋白添加比例为2∶1 时发酵 72 h核苷产量最高.本研究能够为利用植物基原料研发天然发酵调味料提供有益参考.
Abstract
In order to explore the natural fermentation characteristics of Bacillus amyloliquefaciens in plant-based raw materials,the addition concentration of three plant-based raw materials was optimized around bacterial growth and inosine and guanosine production,and the addition ratio of compound plant-based raw materials was determined.At the same time,the sugar consumption,organic acid metabolism,enzyme activity changes and related gene expression differences in the fermentation process of optimal single and compound plant-based raw materials were investigated.The results showed that the yield of nucleosides was the highest and the protease activity of this group was the highest(252.52 U·mL-1)after addition of 50 g·L-1 soybean protein isolate.Wheat gluten was beneficial to Bacillus amyloliquefaciens to have stronger α-amylase activity,but could produce high concentration of acetic acid and inhibit the growth of bacteria.The highest nucleoside yield was obtained when 50 g·L-1 plant-based raw materials and the ratio of gluten and soy protein isolate was 2∶1 after 72 h fermentation.This study can provide a favorable reference for the development of natural fermented seasonings using plant-based raw materials.
关键词
解淀粉芽孢杆菌/植物基原料/核苷/酶活/有机酸Key words
Bacillus amyloliquefaciens/plant-based material/nucleoside/enzyme activity/organic acid引用本文复制引用
基金项目
国家自然科学基金面上项目(31871789)
湖北省工业发酵协同创新中心资助项目(2022KF26)
出版年
2024
NETL
NSTL
万方数据