Preliminary Study on the Characteristics of Nucleoside Production by Bacillus amyloliquefaciens
In order to explore the natural fermentation characteristics of Bacillus amyloliquefaciens in plant-based raw materials,the addition concentration of three plant-based raw materials was optimized around bacterial growth and inosine and guanosine production,and the addition ratio of compound plant-based raw materials was determined.At the same time,the sugar consumption,organic acid metabolism,enzyme activity changes and related gene expression differences in the fermentation process of optimal single and compound plant-based raw materials were investigated.The results showed that the yield of nucleosides was the highest and the protease activity of this group was the highest(252.52 U·mL-1)after addition of 50 g·L-1 soybean protein isolate.Wheat gluten was beneficial to Bacillus amyloliquefaciens to have stronger α-amylase activity,but could produce high concentration of acetic acid and inhibit the growth of bacteria.The highest nucleoside yield was obtained when 50 g·L-1 plant-based raw materials and the ratio of gluten and soy protein isolate was 2∶1 after 72 h fermentation.This study can provide a favorable reference for the development of natural fermented seasonings using plant-based raw materials.
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