超高效液相色谱-串联质谱法测定百合中6种新烟碱类农药残留
Determination of Six Neonicotinoid Pesticide Residues in Lily by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry
冯永刚1
作者信息
- 1. 珠海市食品药品检验所,广东 珠海 519000
- 折叠
摘要
目的:建立固相萃取联合超高效液相色谱-串联质谱法(Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry,UPLC-MS/MS)测定百合中 6 种新烟碱类农药残留的方法.方法:样品经乙腈液提取、PSA固相萃取柱净化后,通过UPLC-MS/MS进行测定.结果:6 种新烟碱类农药在 0.1～10.0 ng·mL-1 范围内线性关系良好,相关系数均大于0.99,方法检出限为0.015～0.250 μg·kg-1,选择3个浓度水平进行回收率实验,平均回收率为 81.5%～117.0%,相对标准偏差为 1.6%～11.3%.结论:该方法简单、快捷,能用于百合中 6 种新烟碱类农药残留的测定.
Abstract
Objective:To establish a method for the determination of six kinds of neonicotinoid pesticide residues in lily by solid phase extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).Method:The samples were extracted by acetonitrile solution,purified by PSA solid phase extraction column,and determined by UPLC-MS/MS.Result:The linear relationship of the six neonicotinoid pesticides was good within the range of 0.1～10.0 ng·mL-1,and the correlation coefficients were all greater than 0.99.The limits of detection is 0.015～0.250 μg·kg-1.Three spiked levels were selected for recovery experiments.The average recovery rates were 81.5%～117.0%,with a relative standard deviation ranging from 1.6%to 11.3%.Conclusion:This simple and rapid method can be used for the determination of six kinds of neonicotinoid pesticide residues in lily.
关键词
新烟碱类农药/百合/超高效液相色谱-串联质谱法/固相萃取Key words
neonicotinoid pesticides/lily/ultra-high performance liquid chromatography-tandem mass spectrometry/solid phase extraction引用本文复制引用
基金项目
广东省药品监督管理局2022年科技创新项目(2022TDB21)
出版年
2024
NETL
NSTL
万方数据