From the point of view of primary seed culture of clostridium butyricum,this study studied the influence of inoculation method,inoculation amount,culture temperature and other culture conditions on culture results.The results show that before the first level inoculation,the bacterial suspension was subjected to 75℃heat treatment and inoculation amount was 4%,the cultivation process was sealed with polypropylene screw caps and incubated at 37℃.After 4 h of cultivation,oscillation was performed each 1 h.The resulting of first level seed culture had higher OD600 value and lower pH.
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