Study on Extraction and Purification Process and Antioxidant Activity of Total Flavonoids from Sea Buckthorn Peel
Objective:To explore the extraction and purification process of total flavonoids from sea buckthorn peel and evaluate its antioxidant activity.Method:Ultrasonic-assisted extraction of total flavonoids from sea buckthorn peel was carried out,and the extraction conditions of flavonoids were optimized by single factor and orthogonal experiments.Crude flavonoids were purified by X-5 macroporous resin,and the antioxidant activities of flavonoids and ascorbic acid were evaluated by comparing their DPPH free radicals and ABTS free radicals scavenging ability.Result:The optimal extraction conditions were solid-liquid ratio 1∶30(g∶mL),ethanol concentration of 50%,extraction time of 70 min,and under these conditions,the extraction yield of flavonoids reached 3.62%.After purification by macroporous resin,flavonoid purity increased from 15.5%to 49.0%,total flavonoid concentration was 1.0 mg·mL-1,and DPPH free radicals clearance rate was 80.37%,ABTS free radicals clearance rate was 77.65%.Conclusion:The purified flavonoids from sea buckthorn fruit peel have certain antioxidant activity and can be used in the development of natural antioxidant.
sea buckthorn peelflavonoidsseparation and purificationantioxidant activity
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