QuEChERS-液相色谱-串联质谱法同时测定代餐食品中8种真菌毒素
Determination of 8 Mycotoxins in Meal Replacement Foods by UPLC-MS/MS Combined with QuEChERS
谢忠阳 1罗诗泳 2苏肯明 2王洪健1
作者信息
- 1. 广东省食品工业研究所有限公司,广东 广州 511442
- 2. 佛山市沃特测试技术服务有限公司,广东 佛山 528000
- 折叠
摘要
目的:建立一种QuEChERS-液相色谱-串联质谱法测定代餐食品中8种真菌毒素的方法.方法:采用1%甲酸-乙腈提取,C18 净化,然后利用高效液相色谱-串联质谱进行检测.结果:8 种真菌毒素在 1.0～50.0 μg·L-1线性关系良好,相关系数(r2)大于 0.990,检出限均为 1.0 μg·kg-1.T-2 毒素回收率为 70.60%～87.71%;HT-2 毒素回收率为 78.35%～84.14%;脱氧雪腐镰刀菌烯醇回收率为 75.00%～91.94%;玉米赤霉烯酮回收率为 71.69%～91.56%;黄曲霉毒素回收率为 72.79%～95.68%.8 种真菌毒素相对标准偏差(Relative Standard Deviation,RSD)为 1.99%～8.00%.结论:本方法结合了QuEChERS前处理的简便性和高效液相色谱-串联质谱的高灵敏度,适用于代餐食品中 8 种真菌毒素的同时测定.
Abstract
Objective:To establish a QuEChERS liquid chromatography tandem mass spectrometry method for the determination of 8 fungal toxins in meal replacement foods.Method:1%formic acid acetonitrile extraction was used,followed by C18 purification and detection using high-performance liquid chromatography tandem mass spectrometry.Result:The 8 fungal toxins showed a good linear relationship between 1.0 μg·L-1 and 50.0 μg·L-1,the correlation coefficient(r2)is greater than 0.990,the limits of detection were 1.0 μg·kg-1.The recoveries of T-2 toxin were 70.60%～87.71%;the recoveries of HT-2 toxin were 78.35%～84.14%;the recoveries of deoxynivalenol were 75.00%～91.94%;the recoveries of zearalenone were 71.69%～91.56%,and the recoveries of aflatoxins were 72.79%～95.68%.The relative standard deviation was 1.99%～8.00%.Conclusion:This method combines the simplicity of QuEChERS pretreatment with the high sensitivity of high-performance liquid chromatography tandem mass spectrometry,and is suitable for the simultaneous determination of 8 fungal toxins in meal replacement foods.
关键词
代餐食品/真菌毒素/QuEChERS/高效液相色谱-串联质谱Key words
meal replacement foods/mycotoxins/QuEChERS/high performance liquid chromatography-tandem mass spectrometry引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据