超高效液相色谱-串联质谱法同时测定水果及其制品中9种禁用色素
Simultaneous Determination of 9 Forbidden Pigments in Fruits and Their Products by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
周春红 1田毅 1刘畅 1盛静 1王春林 1田霞 1赵光铃 1郑霖露 1江雪娇1
作者信息
- 1. 重庆市食品药品检验检测研究院,重庆 409000
- 折叠
摘要
建立一种超高效液相色谱-串联质谱法同时测定水果及其制品中 9 种禁用色素含量的方法.样品用乙腈溶液(80∶20,V∶V)提取,提取液经QuEChERS净化管净化处理,采用Thermo AcclaimTM RSLC120 C18色谱柱(100 mm×2.1 mm,2.2 μm)分离,流动相为乙腈+含 5 mmol·L-1 乙酸铵的 0.1%甲酸水溶液,梯度洗脱,电喷雾正负离子扫描,多反应监测模式检测,外标法定量.结果表明,9 种禁用色素在各自的线性范围内线性关系良好,相关系数r均大于 0.999;方法检出限在 10.12～46.12 μg·kg-1,定量限在 33.70～153.58 μg·kg-1;在 10 μg·kg-1、50 μg·kg-1、100 μg·kg-1 3 个加标水平下,回收率为 82.15%～116.42%,相对标准偏差为 0.35%～4.24%.该方法简单、快捷、准确,适用于水果及其制品中禁用色素非法添加的同时测定.
Abstract
An ultra-high performance liquid chromatography-tandem mass spectrometry method was developed for the simultaneous determination of nine forbidden pigments in fruits and their products.The samples were extracted with acetonitrile solution(80∶20,V∶V),purified by QuEChERS purification tube,and separated by Thermo AcclaimTM RSLC120 C18 Column(100 mm×2.1 mm,2.2 μm),the mobile phase was acetonitrile+0.1%formic acid aqueous solution containing 5 mmol·L-1 ammonium acetate,gradient elution,electrospray positive and negative ion scanning,multiple reaction monitoring mode detection,external standard method quantification.The results showed that the 9 prohibited pigments had a good linear relationship in their respective linear ranges,and the correlation coefficient r was greater than 0.999,and the detection limit of the method was 10.12 μg·kg-1 to 46.12 μg·kg-1,and the quantitative limit was 33.70 μg·kg-1 to 153.58 μg·kg-1.The recoveries were 82.15%to 116.42%and the relative standard deviations were 0.35%to 4.24%at 10 μg·kg-1,50 μg·kg-1 and 100 μg·kg-1.This method is simple,fast and accurate,and is suitable for the simultaneous determination of illegal addition of prohibited pigments in fruits and their products.
关键词
超高效液相色谱-串联质谱/水果及其制品/禁用色素Key words
ultra-high performance liquid chromatography-tandem mass spectrometry/fruits and their products/forbidden pigments引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据