Determination of Dexamethasone Residue in Livestock and Poultry Meat by Isotope Internal Standard Method with High Performance Liquid Chromatography-Tandem Mass Spectrometry
Objective:To develop an analytical method based on high performance liquid chromatography-tandem mass spectrometry for the determination of dexamethasone residues in meat from livestock and poultry.Method:The samples were extracted by 0.2%acetonitrile,purified by ECS hydrolipophilic extraction column,determined by high performance liquid chromatography-tandem mass spectrometry,and quantitatively analyzed by isotope internal standard method.Result:The linear relationship of dexamethasone was good in the range of 0.5～100.0 ng·mL-1 with correlation coefficient more than 0.999,and the limit of quantification was 0.2 μg·kg-1.At the spiked levels of 0.5 μg·kg-1,2.5 μg·kg-1 and 10.0 μg·kg-1,the average recovery was from 92.2%to 99.6%,the relative standard deviation was from 0.6%to 2.5%(n=6).All the methodological indicators meet the technical requirements of GB/T 27404-2008.Conclusion:The method established in this study has the characteristics of simple operation,high accuracy and good repeatability,and is suitable for the determination of dexamethasone residue in livestock and poultry meat.
livestock and poultry meatdexamethasonehigh performance liquid chromatography-mass spectrometryinternal standard method
NETL
NSTL
万方数据
维普
