QuEChERS-气相色谱-三重四极杆质谱法同时测定保健食品中8种N-亚硝胺
Simultaneous Determination of Eight N-nitrosamines in Health Foods by QuEChERS-Gas Chromatography-Triple Quadrupole Mass Spectrometry
徐华伟 1王婷 2程莎2
作者信息
- 1. 青岛市产品质量检验研究院,山东 青岛 266000;青岛市食品检验所,山东 青岛 266000
- 2. 青岛市产品质量检验研究院,山东 青岛 266000
- 折叠
摘要
N-亚硝胺具有强致癌性,保健食品中N-亚硝胺含量的测定鲜有报道.本文利用QuEChERS技术对保健食品中的N-亚硝胺进行提取净化,使用气相色谱-三重四极杆质谱仪分析测试N-亚硝胺类化合物,建立QuEChERS-气相色谱-三重四极杆质谱法同时测定保健食品中 8 种N-亚硝胺的方法.结果表明,8 种N-亚硝胺化合物浓度在 0.5～10.0 μg·L-1 线性良好,线性相关系数均大于 0.999,检出限为 0.12～0.32 μg·kg-1,定量限为 0.40～1.08 μg·kg-1,3 个浓度水平的平均加标回收率为 88.7%～115.4%,相对标准偏差为 2.0%～9.5%.利用该方法对市面上常见的 10 种保健食品进行测定,均未检出 8 种N-亚硝胺.该方法操作简单、取样量少、灵敏度与精密度良好,适用于测定保健食品中的N-亚硝胺化合物.
Abstract
N-nitrosamines have strong carcinogenicity,and there are few reports on the determination of N-nitrosamine content in health food.This paper uses QuEChERS technology to extract and purify N-nitrosamines in health food,and uses gas chromatography-triple quadrupole mass spectrometer to analyze and test N-nitrosamine compounds.The QuEChERS-gas chromatography-triple quadrupole mass spectrometry method is established for the simultaneous determination of eight N-nitrosamines in health food.The experimental results show that the eight N-nitrosamine compounds have good linearity in the concentration range of 0.5 μg·L-1 to 10.0 μg·L-1,with linear correlation coefficients greater than 0.999.The detection limit is from 0.12 μg·g-1 to 0.32 μg·kg-1,and the quantification limit is from 0.40 μg·kg-1 to 1.08 μg·kg-1.The average spiked recovery rates of the three concentration levels is from 88.7%to 115.4%,and the relative standard deviation is from 2.0%to 9.5%.The method is used to determine ten kinds of common health food in the market,and eight kinds of N-nitrosamines are not detected.This method has the advantages of simple operation,small sampling amount,good sensitivity and precision,and is suitable for the determination of N-nitrosamines compounds in health foods.
关键词
N-亚硝胺/保健食品/QuEChERS/气相色谱-三重四极杆质谱法Key words
N-nitrosamine/health food/QuEChERS/gas chromatography-triple quadrupole mass spectrometry引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据