降尿酸乳酸菌的筛选及其特性研究
Screening and Characterization of Uric Acid-Reducing Lactic Acid Bacteria
陈波 1郑智汉 1詹奇奇 1李涛 1高巧婷 1刘辉1
作者信息
- 1. 浙江一鸣食品股份有限公司,浙江 温州 325000
- 折叠
摘要
目的:从天然发酵样本中筛选具有降尿酸潜力的乳酸菌.方法:对分离菌株的核苷降解率、黄嘌呤氧化酶(Xanthine Oxidase,XOD)抑制作用进行测定并筛选,建立高尿酸斑马鱼模型评估菌株降尿酸效果,同时进行菌株组合比例优化.结果:分离筛选出的瑞士乳杆菌GQ1501 可以实现 100%降解肌苷与鸟苷;副干酪乳杆菌R2 和植物乳杆菌D1 对XOD的抑制率分别为 44.05%和 37.61%;高尿酸斑马鱼模型评估得出GQ1501、R2、D1 这 3 株菌株在斑马鱼体内均可以展现出较好的降尿酸效果,其最佳组合比例为R2∶GQ1501∶D1=2∶1∶1,且在给药浓度为 0.5 g·L-1 时,尿酸降解率达到 53.07%;复配菌株可以赋予发酵乳多种风味物质,其中 2-壬酮含量最高,为 86.27 mg·kg-1.结论:本研究分离筛选的菌株及其组合具有开发降尿酸发酵乳的潜力.
Abstract
Objective:To screen lactic acid bacteria with the potential to reduce uric acid from natural fermentation samples.Method:The nucleoside degradation rate and xanthine oxidase(XOD)inhibition of isolated strains were measured and screened.A high uric acid zebrafish model was established to evaluate the uric acid lowering effect of the strains,and the strain combination ratio was optimized.Result:The isolated and screened Lactobacillus helveticus GQ1501 can achieve 100%degradation of inosine and guanosine;the inhibition rates of Lacticaseibacillus paracasei R2 and Lactiplantibacillus plantarum D1 on XOD were 44.05%and 37.61%,respectively;the evaluation of the high uric acid zebrafish model showed that strains GQ1501,R2,and D1 exhibited good uric acid lowering effects in zebrafish.The optimal combination ratio was R2∶GQ1501∶D1=2∶1∶1,and the uric acid degradation rate reached 53.07%at a concentration of 0.5 g·L-1;compound strains can impart various flavor compounds to fermented milk,among which 2-nonanone has the highest content of 86.27 mg·kg-1.Conclusion:The strains and their combinations isolated and screened in this study have the potential to develop uric acid reducing fermented milk.
关键词
乳酸菌/尿酸/核苷/黄嘌呤氧化酶Key words
lactic acid bacteria/uric acid/nucleoside/xanthine oxidase引用本文复制引用
出版年
2024
NETL
NSTL
维普
万方数据