In order to improve the accuracy of the detection results,the matrix effects of 9 pesticides in leek and celery were investigated by standard method GB 23200.121-2021,including acetamidine,imidacloprid,pyrazolate,carbendazim,thianidam,thiamethoxam,enylmorprid,carboxanil and 3-hydroxycarboxanil.Three different brands of high performance liquid series mass spectrometer(LC-MS/MS)were used to determine 9 pesticide components in leek and celery at the same time.The results showed that different brands of instruments showed different degree of matrix effect,and the strength of matrix effect was related to matrix content.By diluting matrix extract,matrix effect could be effectively reduced to ensure the accuracy of data.