Black beans have strong selenium enrichment ability and high protein content,making black bean selenoprotein a high-quality food source for supplementing selenium elements in the human body.In order to improve the extraction rate of selenoprotein from black beans,this study focuses on selenium rich black beans and uses ultrasonic assisted alkaline extraction to extract selenoprotein from black beans.A single factor experiment is designed with the extraction rate of black beans as the indicator to investigate the effects of NaOH concentration,extraction time,solid-liquid ratio,and ultrasonic power on the extraction rate of selenoprotein from black beans.The response surface methodology is used to optimize the extraction process of selenoprotein from black beans.The results showed that the order of the influence of the three factors on the extraction rate of black bean selenoprotein was material to liquid ratio＞NaOH concentration＞extraction time.The optimal extraction conditions for black bean selenium protein are NaOH concentration of 0.16 mol·L-1,extraction time of 4.00 h,solid-liquid ratio of 1∶40,ultrasonic power of 250.00 W.Under these conditions,the extraction rate of black bean selenium protein reaches 59.19％.This study provides a reference for the development of selenium protein resources in black beans.
black beanselenoproteinextraction processresponse surface methodology
维普
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