Optimization of Synchronous and High-Efficient Extraction Process of Anthocyanins and Polyphenols from Perilla Leaves Using Response Surface Methodology
Polyphenols and anthocyanins are the main functional components of Perilla leaf,which give the leaf a variety of biological effects.However,existing studies often optimize extraction for only one of these substances.In this paper,the parameters of simultaneous extraction of polyphenols and anthocyanins from Perilla leaf were obtained by single factor and response surface experiment as extraction temperature 52 ℃,extraction time 48 min and ethanol volume fraction 47%.Under these conditions,the contents of polyphenols and anthocyanins in Perilla leaves were(71.116±5.112)mg·g-1 and(6.323±0.365)mg·g-1,respectively.This paper will provide practical and theoretical reference for reducing the extraction cost and improving the utilization efficiency of active components of Perilla leaf.