Objective:To establish a method for the determination of diclazuril,toltrazuril and their metabolites in animal-derived foods.Method:The samples were extracted with acetonitrile,cleaned up by PSA and detected by liquid chromatography-tandem mass spectrometry.Result:The correlation coefficients(r2)of the four analytes were 0.997 363～0.999 085 in the linear range of 1.0～100.0 μg·L-1.The detection limits of aquatic products,livestock and poultry muscle,and poultry eggs were all 2.5 μg·kg-1,and the detection limits of viscera samples were all 5.0 μg·kg-1.When the concentrations of diclazuril,toltrazuril and their metabolites were added at 12.5～250.0 μg·kg-1,the recoveries in fish samples were 70.01％～88.83％,in chicken samples were 70.60％～89.68％,and in egg samples were 74.83％～88.72％.When the addition concentration was 25.0～500.0 μg·kg-1,the recovery rate of pig liver samples was 70.84％～85.34％,and the relative standard deviation(n=6)was 0.80％～5.35％.Conclusion:This method is simple to operate and has high sensitivity.It is suitable for the determination of diclazuril,toltrazuril and their metabolites in animal-derived foods.
diclazuriltoltrazurifoods of animal originhigh performance liquid chromatography-tandem mass spectrometry
维普
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