Objective:Study the protective effect of compound hepatoprotective beverage prepared from kudzu root,mung bean and other raw materials on chemical liver injury and explore its mechanism.Method:Mice were randomly divided into blank control group,model group,and compound hepatoprotective beverage(3.75,7.50,15.00 g·kg-1)of low,medium and high dose.Acute liver injury model was established by intraperitoneal injection of carbon tetrachloride(CCl4)vegetable oil solution in mice.After the last administration,fasting for 18 hours was performed to measure serum alanine aminotransferase(ALT)activity,liver tissue glutathione(GSH),malondialdehyde(MDA),total superoxide dismutase(T-SOD)levels,and expression levels of Bcl-2 gene and TNF-α gene.Pathological changes in liver tissue were observed.Result:Compared with the model group,the serum ALT level and MDA content were significantly reduced in each dose group,while the GSH content,T-SOD activity,and Bcl-2 gene expression were significantly increased,and the differences were statistically significant(P＜0.05 or P＜0.01).Although the expression level of TNF-α gene was slightly reduced,only the high-dose group showed significant differences and statistical significance(P＜0.05);each dose group has varying degrees of alleviating effects on pathological changes in liver tissue.Conclusion:Compound hepatoprotective beverage has a certain auxiliary protective effect on acute chemical liver injury.
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