In this study,the sources and factors introducing uncertainties in the determination of pentachlorophenol residues in animal derived food by liquid chromatography-tandem mass spectrometry were systematically analysed based on the bottom-up method,and the critical control points affecting the accuracy of detection results were identified and quantified.The results showed that the sample volume and the preparation of a series of standard working solutions were the largest components introducing relative standard uncertainties,which contributed 74.3％of the total.It was found that the use of 15 mL conical centrifuge tubes for sample volume setting and 100 μL pipette for the preparation of standard working solutions were the main factors contributing to the large uncertainty components,which were the critical control points affecting the accuracy of the detection results.The optimization of the critical control points can effectively improve the accuracy of the results.This study can help to improve the accuracy of the determination of pentachlorophenol residues in animal derived food by LC-MS/MS and provides new ideas for analytical quality control.
critical control pointsmeasurement uncertaintypentachlorophenolliquid chromatography-tandem mass spectrometryanimal derived food
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