Objective:To analyze the effects of salinity and temperature on the survival rate and motility of Vibrio parahaemolyticus.Method:Using a single-factor controlled variable method,the survival rate and colony diameter of Vibrio parahaemolyticus were measured under different temperatures and salinities.The experimental results were subjected to single-factor analysis of variance and multiple comparison analysis.Result:The study found that the temperature and salinity significantly affected the survival rate and motility of Vibrio parahaemolyticus.The highest survival rate and motility were observed at a temperature of 36℃.Below 15 ℃,the bacteria ceased to grow and showed no motility.The optimal salinity for survival rate was 3%,while the best motility was recorded at 4.5%salinity.The survival rate and motility were significantly higher than other experimental groups at 3.0%and 4.5%salinity(P<0.01).Conclusion:Temperature and salinity are crucial factors in regulating the survival rate and motility of Vibrio parahaemolyticus.